California State University, Los Angeles

Dr.DARMANYAN

Chemistry 353

Nutritional Aspects of Biochemistry

SYLLABUS

Course objectives
This course is designed for nutrition majors to introduce the basic concepts of biochemistry, including classification, structure, and function of biological molecules; the role of major metabolic pathways, and the nature of genetic material with particular emphasis on the understanding of the metabolism and function of nutrients.

Prerequisites
Satisfactory completion (C or better) of Chem.152 or equivalent, presuming an understanding of the organic chemistry of biologically important substances, concepts of chemical bonding, functional groups, dissociation constants, acid-base properties, pH, buffers, oxidation-reduction reactions, and stereochemistry

Class Schedule
Lecture hours: Tuesday, Thursday 9:50 AM – 11:30 AM SH-C- 162

Contact the Instructor
Tel: (323) 343-2300 to leave messageE-mail: pdarmany@lausd.netOffice hours: TBA

Lecture Materials
Textbook: Hill, J.W., Baum, S.J., and Scott-Ennis, R.J. Chemistry and Life, 6th Ed. Prentice Hall, 2000.(ISBN# 0-13-082181-0)
Supplementary textbook (recommended): Hackett, M.L., Sandwick, R.K., Pelter, M.W., Pelter, L.S.Study Guide and Solutions Manual. 6th Ed. Prentice Hall, 2000.
Laboratory MaterialsLaboratory Manual: Horowitz, S.T. Laboratory Manual for Biochemistry 353 (revised 1999)Required supplies: safety goggles (see details under “Laboratory” section)

Grading (pts)
Review quiz - 30
Midterm exams (2x210) - 420
Laboratory - 300
Final exam - 250
Total -1000

The point score corresponds to the final letter grades:
91.0-100 % - A
89.5-90.9 % - A-
88.0-89.4 % - B+
80.0-87.9 % - B
78.5-79.9 % - B-
77.0-78.4% - C+
67.0-76.9 % - C
65.0-66.9 % - C-
55.0-64.9 % - D
0-54.9 % - F

Midterm Exams and Quiz
Graded exams/quiz will be available for you to look at the following week. If you need further discussion of your exam/quiz, arrange time during office hours. Your exam/quiz paper should be returned to the instructor after review. Exams/quizzes are not to leave the classroom. Your exam/quiz score will be recorded after the test/quiz has been reviewed by the student. Exams/quizzes not returned will be recorded as zero for that exam/quiz.There will be no makeup exams or quiz. Missed assignments will be counted as zero, exceptextenuating circumstances, such as illnesses that must be certified by a signed note from a doctor.Homework AssignmentsHomework will not be collected. To keep up in the lecture material, answer all in-chapter questions on that topic. Answer the assigned end-of-chapter questions and self-help tests in the Study Guide pertaining to the material covered.

Tentative Lecture Schedule

Week - Lecture - Text chapter

Domain I. Classification, Structure and Functions of Biological Molecules
1. Introduction. Carbohydrates: Monosaccharides
Carbohydrates: Di- and Polysaccharides Ch.19

2. Review Quiz. Lipids (I)
Lipids (2) Ch.20

3. Amino acids.
Peptides and Proteins Ch.21

4. Midterm Exam 1

Domain II. Metabolism and Energy

Enzymes Ch.22

5. Enzyme cofactors. Vitamins Ch.22
Nucleic acids and Protein synthesis Ch.23

6. Digestion and Absorption of Major Nutrients Ch.23
Metabolism: Energy Production Ch.24

7. Midterm Exam 2
Carbohydrate Metabolism (1) Ch.25

8. Carbohydrate Metabolism (2) Ch.25
Lipid Metabolism (I) Ch.26

9. Lipid Metabolism (2) Ch.26
Protein Metabolism (I) Ch.27

10.Protein Metabolism (2)
Nutritional Genomics Ch.27

11. Final Exam

Chem 353 Laboratory Required Materials:

1. A bound laboratory notebook for recording data

2. Safety glasses (prescription glasses are not sufficient)

3. A breakage card from the Cashier’s Office (this must be turned in to the chemistry stockroom by the end of the first week of class; you will not be issued a locker combination without it).

Lab grading (pts):

Laboratory Reports - 150
Pre-lab write ups and lab participation - 50
Quizzes (10 pts x 5 ) - 50
Final lab exam - 50
Total - 300

Other important lab information:
A pre-lab write up in your laboratory notebook should be done for each experiment before that lab session. Pre-labs will be checked during recitation and are worth 5 pts each. Attendance in the laboratory section is mandatory, and points will be deducted for each laboratory session missed. All lab reports are due the week following completion of the experiment. Lab reports are marked down by 10% for each working day they are late. There are no make-up quizzes.

Laboratory schedule

Week - Experiment - Lab report, pts
1. Introduction, check-in

2. Carbohydrates:
Exp.V. Identification of two unknowns 15

3. Carbohydrates:
Identification of two unknowns (cont.) 15

4. Lipids:
Exp. VI. Cholesterol assay
Exp. IV. Directions for the Spectrophotometer 20

5. Amino acids and Proteins:
Exp.1. Denaturation of proteins
Amino acid electrophoresis (dry lab) 20

6. Exp. IV. Biuret assay of proteins 20

7. Enzymes: Exp. III. Potato polyphenol oxidase 20

8. Exp. VIII. Protein biosynthesis.
Exp. II. Electrophoresis of hemoglobin 20

9. Protein biosynthesis.
Exp. VIII. DNA electrophoresis (Handout) 20

10. Clean-up, check-out